Kinetic analysis of experiments involving the single turnover of an enzyme.
نویسندگان
چکیده
The complete solution to the kinetic equation for nucleotide fluorescence quenching on addition of pyruvate to the late dehydrogenase-NADH complex modifies previous interpretations of such experiments.
منابع مشابه
Coordinating Role of His216 in MgATP Binding and Cleavage in Pyruvate Carboxylase
His216 is a well-conserved residue in pyruvate carboxylases and, on the basis of structures of the enzyme, appears to have a role in the binding of MgATP, forming an interaction with the 3'-hydroxyl group of the ribose ring. Mutation of this residue to asparagine results in a 9-fold increase in the Km for MgATP in its steady-state cleavage in the absence of pyruvate and a 3-fold increase in the...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 157 1 شماره
صفحات -
تاریخ انتشار 1976